Morphology and Some Histochemical Peculiarities of the Mucous Glands in the Integument of the Green Toads of Bufo viridis Complex (Amphibia: Bufonidae)

Tatjana N. Dujsebayeva, Irina I. Arifulova, Galina V. Fedotovskikh


We described three groups (varieties) of the mucous glands in the skin of the green toads of Bufo viridis complex basing on the morphology and histochemistry [mucopolycaccharide (MPS) composition] of their secretory epithelium and a localization of the glands in the toad integument. Our study showed that the mucous cells of three gland groups were differed in their morphology and content of the acid and neutral MPS. Only the ordinary mucous glands (group I) were found within the entire integument and only the skin of parotoid complexes contained the mucous glands of all three varieties. We consider the glands of group III as structures of full secretory maturity. Morphological and histochemical features of the glands of II (and possibly III) varieties as well as their selective distribution in the integument of the green toads permit to suppose their specific functions which need to be elucidate.


Amphibia; Bufo viridis complex; integument; mucous glands; distribution; morphology; histochemistry; mucopolysaccharides

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Arifulova I. I., Dujsebayeva T. N., and Nurtazin S. T. (2007) «Morphological peculiarities of the skin of the Green Toads (Anura: Bufonidae: Bufo viridis complex)», Vestnik KazNU. Ser. Biol., 3(33), 127 – 136 [in Russian with English summary].

Blaylock L. A., Ruibal R., and Platt-Aloia K. (1976), «Skin structure and wiping behavior of phyllomedusine frogs», Copeia, 1976(2), 283 – 295.

Bovbjerg A. M. (1963), «Development of the glands of the dermal plicae in Rana pipiens», J. Morphol., 113, 231 – 243.

Brizzi R., Delfino G., and Jantra S. (2005), «Structure and functional significance of the nuptial thumb pads in Rana esculenta and R. perezi», in: Herpetologia Petropolitana, N. Ananjeva and O. Tsinenko (eds.), pp. 258 – 262.

Castellano S., Giacoma C., Dujsebaeva T., Gaetano I., and Balletto E. (1998), «Morphometrical and acoustical comparison between diploid and tetraploid green toads», Biol. J. Linn. Soc., 63, 257 – 281.

Duellman W. E. and Trueb L. (1986), Biology of Amphibians, McGraw–Hill, New York.

Fox H. (1986), «The skin of Amphibia», in: Biology of the Integument. Vol. 2. Vertebrates, J. Bereiter-Hahn, A. G. Matoltsy, and K. S. Richards (eds.), Springer Verlag, Heidelberg, pp. 78 – 135.

Fox H. (1995), «The structure of the integument», in: Amphibian Biology. Vol. I. Integument, H. Heatwole and G. T. Barthalmus (eds.), Surrey Beathe & Sons, Australia, pp. 1 – 32.

Fujikura K., Kurabuchi S., Tabuchi M., and Inoue S. (1988), «Morphology and distribution of the skin glands in Xenopus laevis and their response to experimental stimulations» Zool. Sci., 5, 415 – 430.

Hein K. and Sinsch U. (1995), «Histological investigations on the skin structure of Neotropical Water Frogs (Leptodactylidae: Telmatobiinae)», in: Abstrs. of the 8th Ord. Gen. Mit. Soc. Eur. Herpetol., August 23 – 27, 1995, Bonn, Germany, pp. 62.

Horobin R. W. and Kewill-Davies I. M. (1971), «A mechanistic study of the histochemical reaction between aldegides and Basic Fucshin in acid alcohol used as a simplified to schiffis reagent», Histochem. J., 3(5), 371 – 378.

Kent G. C. (1991), Comparative Anatomy of Vertebrates, Mosby Year Book, St. Luis – Baltimore – Boston – Chicago – London – Philadelphia – Sydney – Toronto.

Kurosumi K. (1961), «Electron microscopic analysis of the secretion mechanism», Int. Rev. Cytol., 2, 1 – 117.

Martoja R. and Martoja–Pierson M. (1967), Initiation oux Techniques de lHistologie Animale, Masson, Paris.

McDowell W. T. and Martan J. (1994), «Skin glands of Hynobius nigrescens and Onychodactylus japonicus (Caudata: Hynobiidae)», J. Herpetol., 28(4), 506 – 508.

Noble G. A. and Noble E. R. (1944), «On the histology of frog skin glands», Trans. Am. Microsc. Soc., 63, 254 – 263.

Romeis B. (1954), Microscopic Technics, Moscow [in Russian].

Sever D. M. (1989), «Caudal hedonic glands in salamanders of the Eurycea baslineata complex (Amphibia: Plethodontidae)», Herpetologica, 45(3), 322 – 329.

Shubnikova E. A. (1967), Cytology and Cytophysiology of the Secretory Process (Secretory Cell), Izd. MGU, Moscow [in Russian].

Sjöber E. and Flock Å. (1976), «Innervation of skin glands in the frog», Cell Tissue Res., 172, 81 – 94.

Sokolov V. E., Chernova O. F., and Salomatina N. I. (1988), «Chemical communication in Amphibia», in: V. E. Sokolov (ed.), Itogy Nauki i Tekhniki. Zoologiya pozvonochnykh [The Results of Sciences and Technics. Vertebrate Zoology]. Vol. 15. Ékologicheskiye i Étologicheskiye Aspekty Chimicheskoy Kommunikatsii Nasemnykh Pozvonochnykh [Ecological and Ethological Aspects of Communication in Terrestrial Vertebrates], Moscow, pp. 5 – 91 [in Russian].

Stebbins R. C. and Cohen N. W. (1995), A Natural History of Amphibians, Princeton Univ. Press, Princeton.

Thomas E. O, Tsang L., and Light P. (1993), «Comparative hystochemistry of the sexually dimorphic skin glands of anuran amphibians», Copeia, 1993(1), 133 – 143.

Whitear M. (1955), «Dermal nerve endings in Rana and Bufo», Q. J. Microsc. Sci., 96, 343 – 349.

Whitear M. (1974), «The nerves in frog skin», J. Zool. Lond., 172, 503 – 529.

Wilber C. G. and Carrol P. L. (1940), «Studies on the histology of the glands in the skin of Anura. I. The paratoid gland of Bufo americanus Holbrook», Trans. Am. Microsc. Soc., 59, 123 – 128.



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