Cryobanking of Amphibian Genetic Recourses in Russia: Past and Future

Victor K. Uteshev, Edith N. Gakhova, Ludmila I. Kramarova, Natalia V. Shishova, Svetlana A. Kaurova, Robert K. Browne

Abstract


In modern biology, a search for efficient and safe ways of long-term storage of animal genomes is vital for the survival of rare and endangered species. To date, the only reliable method of prolonging the preservation of genome is deep freezing of somatic and reproductive cells, including spermatozoa. Here we overview the current state of reproductive technologies and present the cryopresevation strategies of genetic material of selected amphibian species. These strategies were developed at the Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia. Cryopreservation could help managing the threatened populations of amphibian species through genome storage and mediate the production of healthy animals from the stored material.

Keywords


cryobanking; cryopeservation; amphibian; reproductive material

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References


Ananjeva N. B., Uteshev V. K., Orlov N. L. and Gakhova E. N. (2015), «Strategies for Conservation of Endangered Amphibian and Reptile Species», Biol. Bull., 42(5), 432 – 439.

Ananjeva N. B., Uteshev V. K., Orlov N. L., Ryabov S. A., Gakhova E. N., Kaurova S. A., Kramarova L. I., Shishova N. V., Browne R. K. (2017), «Comparison of the modern reproductive technologies for amphibians and reptiles», Russ. J. Herpetol., 24(4), 275 – 290.

Browne R. K., Clulow J., Mahony M., and Clark A. (1998), «Successful recovery of motility and fertility of cryopreserved cane toad (Bufo marinus) sperm», Cryobiology, 37, 339 – 345.

Browne R. K., Clulow J., and Mahony M. (2002a), «The short-term storage and cryopreservation of spermatozoa from hylid and myobatrachid frogs», Cryol. Lett., 23, 129 – 136.

Browne R. K., Davis J., Pomering M., and Clulow J. (2002b), «Storage of cane toad (Bufo marinus) sperm for 6 days at 0 with subsequent cryopreservation», Reprod. Fertil. Devel., 14(5), 267 – 273.

Browne R. K., Li H., Robertson H., Uteshev V. K., Shishova N. V., McGinnity D., Nofs S., Figiel C. R., Mansour N., Lloyd R. E., Agnew D., Carleton C. L., Wu M., and Gakhova E. N. (2011), «Reptile and amphibian conservation through gene banking and other reproduction technologies», Russ. J. Herpetol., 18(3), 165 – 174.

Clulow J., Trudeau V. L., and Kouba A. J. (2014), «Amphibian declines in the twenty-first century: why we need assisted reproductive technologies», in: W. V. Holt, J. L. Brown, and P. Comizzoli (eds.), Advances in Experimental Medicine and Biology. Vol. 753, pp. 275 – 316. .

Clulow J. and Clulow S. (2016), «Cryopreservation and other assisted reproductive technologies for the conservation of threatened amphibians and reptiles: bringing the ARTs up to speed», Reprod. Fertil. Devel., 28(8), 1116 – 1132.

Gakhova E. N. (1998), «Genetic cryobanks for conservation of biodiversity. The development and current status of this problem in Russia», Cryol. Lett., Suppl. 1, 57 – 64.

Gakhova E. N., Uteshev V. K., Shishova N. V., and Yashina S. G. (2006), «Genetic resource bank for animal and plant species in Institute of cell biophysics of RAS», Biofiz. Zhiv. Kletki, 8, 14 – 38 [in Russian].

Kaurova S. A., Chekurova N. R., Melnikova E. V., Uteshev V. K., and Gakhova E. N. (1996), «Cryopreservation of frog Rana temporaria sperm without loss of fertilizing capacity», in: Genetic Resource Conservation. Proc. of XIV Working Meeting, May 13 – 15, 1996, Pushchino, pp. 106 – 108 [in Russian].

Kaurova S. A., Uteshev V. K., Chekurova N. R., and Gakhova E. N. (1997), «Cryopreservation of testis of frog Rana temporaria», Infusionsther. Transfusionsmed., 24(5), 78 – 79.

Kouba A. J. and Vance C. K. (2009), «Applied reproductive technologies and genetic resource banking for amphibian conservation», Reprod. Fertil. Devel., 21(6), 719 – 737.

Kouba A., Vance C., Calatayud N., Rowlison T., Langhorne C., and Willard S. (2012), «Assisted reproductive technologies (ART) for Amphibians», in: V. A. Poole (ed.)., Amphibian Husbandry Resource Guide. 2nd Edition, National Aquarium in Baltimore and Shelly Grow, Association of Zoos and Aquariums, Baltimore, pp. 60 – 118.

Kouba A. J., Lloyd R. E., Houck M. L., Silla A. J., Calatayud N., Trudeau V. L., Clulow J., Molinia F., Langhorne C., Vance C., Arregui L., Germano J., Lermen D., and Della Togna G. (2013), «Emerging trends for biobanking amphibian genetic resources: the hope, reality and challenges for the next decade», Biol. Conserv., 164, 10 – 21.

Mansour N., Lahnsteiner F., and Patzner R. A. (2009), «Optimization of the cryopreservation of African clawed frog (Xenopus laevis) sperm», Theriogenology, 72, 1221 – 1228.

Mansour N., Lahnsteiner F., and Patzner R. A. (2010), «Motility and cryopreservation of spermatozoa of European common frog, Rana temporaria», Theriogenology, 74, 724 – 732.

Mansour N., Lahnsteiner F., and Patzner R. A. (2011), «Collection of gametes from live axolotl, Ambystoma mexicanum, and standardization of in vitro fertilization», Theriogenology, 75, 354 – 361.

Melnikova E. V., Kaurova S. A., Uteshev A., Nikitin V. A., and Gakhova E. N. (2003), «Viability control of the cryopreserved cells of dissociated amphibian embryos», Biofiz. Zhiv. Kletki, 7, 70 – 73 [in Russian].

Melnikova E. V., Uteshev V. K., Pashovkin T. N., Sokolova A. A., Yashin V. A., Karnaukhov V. N., and Gakhova E. N. (2005), «Change in the fluorochrome permeability of amphibian embryonic membranes under the action of ultrasound», Biofizika, 50(3), 441 – 444.

Shishova N. V., Uteshev V. K., Kaurova S. A., Browne R. K., and Gakhova E. N. (2011), «Cryopreservation of hormonally induced sperm for the conservation of threatened amphibians with Rana temporaria as a model research species», Theriogenology, 75(2), 220 – 232.

Uteshev V. K. and Gakhova E. N. (1994), «The prospects of amphibian gene cryobank creation», Biofiz. Zhiv. Kletki, 6, 28 – 34 [in Russian].

Uteshev V. K., Melnikova E. V., Kaurova S. A., Nikitin V. A., Gakhova E. N., and Karnaukhov V. N. (2002), «Fluorescent analysis of cryopreserved totipotent cells of amphibian embryos», Biofizika, 47(3), 539 – 545.

Uteshev V. K. and Gakhova E. N. (2005), «Gene cryobanks for conservation of endangered amphibian species», in: N. Ananjeva and O. Tsinenko (eds.), Herpetologia Petropolitana, Proc. of the 12th Ord. Gen. Meeting of the Soc. Eur. Herpetol., 12 – 16 August 2003, St. Petersburg, Russia, pp. 233 – 234.

Uteshev V. K., Pashovkin T. N., Sevirov A. N., Melnikova E. V., Sadikova D. G., Karnaukhov V. N., and Gakhova E. N. (2006), «The survival of amphibian embryos after continuous ultrasonic treatment», Biofizika, 51(3), 484 – 488.

Uteshev V. K., Shishova N. V., Kaurova S. A., Manokhin A. A., and Gakhova E. N. (2013), «Collection and cryopreservation of hormonally induced sperm of pool frog (Pelophylax lessonae)», Russ. J. Herpetol., 20(2), 105 – 109.

Uteshev V. K., Kaurova S. A., Shishova N. V., Stolyarov S. D., Browne R. K., and Gakhova E. N. (2015), «In vitro fertilization with hormonally induced sperm and eggs from sharp-ribbed newts Pleurodeles waltl», Russ. J. Herpetol., 22(1), 35 – 40.

Uteshev V. K., Kaurova S. A., Shishova N. V., Kramarova L. I., Browne R. K., and Gakhova E. N. (2015), «Current reproductive and cryopreservation technologies in herpetology», Russ. J. Herpetol., 22(2), 143 – 144.

Uteshev V. K., Gakhova E. N., Kaurova S. A., Kramarova L. I., and Shishova N. V. (2016), «Modern reproductive technologies for amphibian biodiversity preservation», Vestn. SPbU Ser. 3. Biol., 3, 157 – 164 [in Russian].

Veprintsev B. N. and Rott N. N. (1979), «Conserving genetic resources of animal species», Nature, 280(5724), 633 – 634.




DOI: https://doi.org/10.30906/1026-2296-2019-26-6-319-324

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