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Morphometric Analysis and Characterization of Peripheral Blood Cells in the Golden Tree Snake Chrysopelea ornata (Shaw, 1802)

Theerakamol Pengsakul, Sinlapachai Senarat, Woranop Sukparangsi, Koraon Wongkamhaeng, Pisit Poolprsert, Sansareeya Wangkulangkul, Jes Kettratad, Wannee Jiraungkoorskul

Abstract


Reptile hematology provides important information to help assessment of health conditions and other reptile physiology and for biomarker discovery. The current hematology of snake from natural habitats of Thailand with morphological measurement is still rarely explored. In the present study, the extensive investigation on the morphometric analysis and characteristics of blood cells in the golden tree snake Chrysopelea ornata (Shaw, 1802) was examined and compared between sexes. Here we found that the majority of the squamate blood cells were erythrocyte in both sexes of this snake. It had the typical erythrocyte morphology showing eclipse shape with a central nucleus without significant difference between males and females. White blood cells (or leukocytes) in the snakes were a heterogeneous group with different nucleus shape and also shared a similar size between sexes. Interestingly, the length of thrombocytes was significantly different (P < 0.05): longer in the male and shorter in the female snakes. From the blood smear technique, the heterogeneous group of the snake leukocyte included azurophil, heterophils neutrophil, basophil, lymphocyte, and monocyte without eosinophil. Remarkably, the highest proportion of the snake leukocyte was lymphocyte, followed by azurophils, and neutrophil. This proportion was similar in both sexes. The results from our hematological analysis could be provided as hematology-based health monitoring of the wild population for C. ornata in our land and other countries.

Keywords


blood cell; erythrocyte; golden tree snake; morphometric analysis; Thailand; white blood cell

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References


Alleman A. R., Jacobson E. R., and Raskin R. E. (1999), «Morphologic, cytochemical staining, and ultrastructural characteristics of blood cells from eastern diamondback rattlesnakes (Crotalus adamanteus)», Am. J. Vet. Res., 60(4), 507 – 514.

Allender M. C., Mitchell M. A., Phillips C. P., Gruszynski K., and Beasley V. R. (2006), «Hematology, plasma biochemistry, and antibodies to select viruses in wild-caught eastern Massasauga rattlesnakes (Sistrurus catenatus catenatus) from Illinois», J. Wild. Dis., 42(1), 107 – 114.

Arikan H., Gocmen B., Atatur M. K., Kumluta Y., and Cicek K. (2009), «Morphology of peripheral blood cells from various Turkish snakes», North-West J. Zool., 5(1), 61 – 73.

Azevedo A. and Lunardi L. O. (2003), «Cytochemical characterization of eosinophilic leukocytes circulating in the blood of the turtle (Chrysemys dorbignih)», Acta Histochem., 105(1), 99 – 105.

Bell K. A. H. and Gregory T. (2014), «White blood cells in Northwestern Garter snakes (Thamnophis ordinoides)», Herpetology, 7, 535 – 541.

Bloom J. C. and Brandt J. T. (2008), «Toxic responses of the blood», in: C. D. Klaassen (ed.), Casarett and Doull’s Toxicology: The Basic Science of Poisons. 7th Edition, McGraw-Hill, New York, pp. 455 – 484.

Cabagna M. C., Lajmanovich R. C., Stringhini G., Sanchez Hernandez J. C., and Peltzer P. M. (2005), «Hematological parameters of health status in the common toad Bufo arenarum in agroecosystems of Santa Fe Province, Argentina», Appl. Herpetol., 2(4), 373 – 380.

Campbell T. (1998), «Interpretation of the reptilian blood profile», Exot. Pet Pract., 3(5), 33 – 34.

Campbell T. W. (2006), «Clinical pathology of reptiles», in: D. R. Mader (ed), Reptile Medicine and Surgery, Saunders Elsevier, Philadelphia, pp. 453 – 470.

Campbell T. and Murru F. (1990), «An introduction to fish hematology», Compend. Contin. Educ. Pract. Vet., 12(4), 525 – 532.

Canfield P. J. (1998), «Comparative cell morphology in the peripheral blood film from exotic and native animals», Aust. Vet. J., 76(12), 793 – 800.

Clark P., Johnstone A. C., Ellison R., and Goold M. (2001), «Inclusions in the erythrocytes of eastern water dragons (Physignathus lesueurii)», Aust. Vet. J., 79(1), 61 – 62.

Chung C. S., Cheng C. H., Chin S. C., Lee A. H., and Chi C. H. (2009), «Morphologic and cytochemical characteristics of Asian yellow pond turtle (Ocadia sinensis) blood cells and their hematologic and plasma biochemical reference values», J. Zool. Wild. Med., 40(1), 76 – 85.

Dessauer H. C. (1970), «Blood chemistry of reptiles: physiological and evolutionary aspects», in: C. Gans and T. Parsons, Biology of the Reptilia, Acad. Press, New York, pp. l – 72.

Davis A. K., Maney D. L., and Maerz J. C. (2008), «The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists», Funct. Ecol., 22(5), 760 – 772

Dotson T. K., Ramsay E. R., and Bounous D. I. (1995), «A color atlas of blood cells of the yellow rat snake», Compend. Contin. Educ. Pract. Vet., 17, 1013 – 1016.

Dissanayake D. S. B., Thewarage L. D., Rathnayake M. P., Rathnayake M., Senanayake A. K. M., Jamburagoda G., and Rajapakse P. V. (2017), «Hematological and plasma biochemical parameters in a wild population of Naja naja (Linnaeus, 1758) in Sri Lanka», J. Venom. Anim. Toxins Incl. Trop. Dis., 23(8), 1 – 9.

Duguy R. (2010), «Numbers of blood cells and their variations», in: C. Gans and T. Parsons, Biology of the Reptilia, Acad. Press, New York, pp. 93 – 109.

Frye F. L. (1991), «Hematology as applied to clinical reptile medicine», in: F. L. Frye, Biomedical and Surgical Aspects of Captive Reptile Husbandry. 2nd Edition, Krieger Publishing Co, Malabar, pp. 209 – 280.

Javanbakht H., Vaissi S., and Parto V. P. (2013), «The morphological characterization of the blood cells in the three species of turtle and tortoise in Iran», Res. Zool., 3(1), 38 – 44.

Godfrey S. S., Nelson N. J., and Bull C. M. (2011), «Ecology and dynamics of the blood parasite, Hepatozoon tuatarae (Apicomplexa), in tuatara (Sphenodon punctatus) on Stephens Island, New Zealand», J. Wildl. Dis., 47(1), 126 – 139.

Heard D., Harr K., and Wellehan J. (2004), «Diagnostic sampling and laboratory tests», in: S. J. Girling, and P. Raiti (eds.), BSAVA Manual of Reptiles. 2nd Edition, Blackwell Publishing, United Kingdom, pp. 78 – 79.

Haklová B., Majláthová V., Majláth I., Harris D. J., Petrilla V., Litschka-Koen T., Oros M., and Peťko B. (2014), «Phylogenetic relationship of Hepatozoon blood parasites found in snakes from Africa», America. Asia, 141(3), 389 – 398.

Hodges R. D. (1977), «Normal avian (poultry) haematology», in: R. K. Archer and L. B. Jeffcott (eds.), Comparative Clinical Haematology, Blackwell Scientific, Oxford, pp. 483 – 517.

Hutchison V. H. and Szarski H. (1965), «Number of erythrocytes in some amphibians and reptiles», Copeia, 1965(3), 371 – 375.

IBM Corp. (2017), IBM SPSS Statistics for Windows. Ver. 25.0, IBM Corp., Armonk, NY.

Lay P. A. and Baldwin J. (1999), «What determines the size of teleost erythrocytes? Correlations with oxygen transport and nuclear volume», Fish Physiol. Biochem., 20, 31 – 35.

Laurie J., Janalee V., and Caldwell P. (2013), Herpetology: An Introductory Biology of Amphibians and Reptiles, https://www.elsevier.com/books/herpetology/vitt/978-0-12-386919-7 (accessed on April 1, 2020).

Martínez-Silvestre A., Mateo J. A., and Silveira L. S. (2001), «Presencia de protozoos intraeritrocitarios en el lagarto gigante de La Gomera (Gallotia simonyi gomerana)», Bol. Asoc. Herpetol. Esp., 12(2), 90 – 92.

Mayanskii A. N. and Pikuza O. I. (1993), The Clinical Aspects of Phagocytosis, Magarif, Kazan [in Russian].

Metin K., Türkozan O., Kargin F., Koca Y. B., Taskavak E., and Koca S. (2006), «Blood cell morphology and plasma biochemistry of the captive European pond turtle Emys orbicularis», Acta Vet. Brno, 75(1), 49 – 55.

Nurngsomsri P. (2015), «Geographic distribution: Chrysopelea ornata (Golden tree snake)», Herpetol. Rev., 45(2), 284 – 285.

Oliveira P. M. A. (2003), Animais Silvestres e Exóticos na Clínica Particular, Roca, Săo Paulo, pp. 332 – 333.

Oliveira-Júnior A. A., Tavares-Dias M., and Marcon L. (2009), «Biochemical and hematological reference ranges for Amazon freshwater turtle, Podocnemis expansa (Reptilia: Pelomedusidae), with morphologic assessment of blood cells», Res. Vet. Sci., 86(1), 146 – 151.

Quadrini A. E., Garcia B. C., Freire B. C., and Martins M. F. M. (2018), «Haematological reference of snakes: Amazon tree boa (Corallus hortulanus, Linnaeus, 1758) and Burmenese Python (Python bivittatus, Kuhl, 1820) in captive», Arq. Bras. Med. Vet. Zool., 70(4), 1172 – 1178.

Ozzetti P. A., Cavlac C. L., and Sano-Martins I. S. (2013), «Hematological reference values of the snakes Oxyrhopus guibei and Xenodon neuwiedii (Serpentes: Dipsadidae)», Comp. Clin. Pathol., 24(1), 101 – 108.

Saint-Girons M. C. (1970), «Morphology of the circulating blood cells», in: C. Gans and T. S. Parsons (eds.), Biology of the Reptilia. 3rd Edition, Acad. Press, New York, pp. 73 – 91.

Sano-Martins I. S. (1978), Hematologia comparada de serpentes Bothrops jararaca e Waglerophis merremii, Universidade de S. Paulo, S. Paulo.

Salakij C., Salakij J., Apibal S., Narkkong N., Chanhome L., and Rochanapat N. (2002), «Hematology, morphology, cytochemical staining and ultrastructural characteristics of blood cells in King cobras (Ophiophagus hannah)», Vet. Clin. Pathol., 31(3), 116 – 126.

Serpunin G. G. and Korobeinikova E. G. (1997), «Response of the blood system of carp (Cyprinus carpio L.) to the effects of heavy metals», in: Proc. of the 1st Congr. of Ichthyologists of Russia NPO BIOS, Astrakhan, pp. 237 – 238 [in Russian].

Shutler D. and Marcogliese D. J. (2011), «Leukocyte profiles of Northern Leopard Frog, Lithobates pipiens, exposed to pesticides and hematozoa in agricultural wetlands», Copeia, 2011(2), 301 – 307.

Smith H. M. (1925), «Cell size and metabolic activity in Amphibia», Biol. Bull., 48(5), 347 – 378.

Stacy N. I., Alleman A. R., and Sayler K. A. (2011), «Diagnostic hematology of reptiles», Clin. Lab. Med., 31(2011), 87 – 108.

Strik N. I., Alleman A. R., and Harr K. E. (2007), «Circulating inflammatory cells», in: E. R. Jacobson (ed.), Infectious Diseases and Pathology of Reptiles: Color Atlas and Text. 1st Edition, CRC Press, Boca Raton (FL), pp. 167 – 218.

Vasaruchapong T., Disarapong P., Chulasugandha P., Khow O., Chanhome L., Chiobamroongkiat M., Chaiyabutr N., and Sitprija V. (2013), «Comparative studies on hematological and plasma biochemical parameters in different types of venomous snakes in Thailand», Comp. Clin. Pathol., 23(4), 955 – 959.

Vitt L. G. and Caldwell J. P. (2014), Herpetology: An Introductory Biology of Amphibians and Reptiles. 4th Edition, Acad. Press, New York.

Watson C. F., Baer K. N., and Benson W. H. (1989), «Dorsal gill incision: a simple method for obtaining blood samples in small fish», Environ. Toxicol. Chem., 8(5), 457 – 461.

Weinstein S. A., Warrell D. A., White J., and Keyler D. E. (2011), Venomous Bites from Non-Venomous Snakes: A Critical Analysis of Risk and Management of «Colubrid» Snake Bites, Elsevier, London.

Wojtaszek J. S. (1991). «Hamatology of the grass snake Natrix natrix natrix L»., Comp Biochem. Physiol., 100(4), 805 – 812.

Work T. M., Raskin R. E., Balazs G. H., and Whittaker S. D. (1998), «Morphological and cytochemical characteristics of blood cells from Hawaiian green turtles», Am. J. Vet. Res., 59(10), 1252 – 1257.

Xu H., Zhang X., Li H., Li C., Huo X. J., Hou L. P., and Gong Z. (2018), «Immune response induced by major environmental pollutants through altering neutrophils in zebrafish larvae», Aqua. Toxicol., 201, 99 – 108.

Zhelev Z., Arif M., Popgeorgiev G., Rauf M., and Mehterov N. (2014), «Haematology of Pelophylax ridibundus (Amphibia: Ranidae) of striata and maculata morphs in populations living in conditions of anthropogenic pollution in Southern Bulgaria», Open J. Anim. Sci., 4(4), 206 – 216.




DOI: https://doi.org/10.30906/1026-2296-2023-30-1-11-19

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