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Trophic Ecology of Post-Metamorphic Dendropsophus minutus (Peters, 1872) in Coastal Wetlands of Southern Brazil (Hylidae: Dendropsophini)

Sônia Huckembeck, Raúl Maneyro, Daniel Loebmann, Alexandre Miranda Garcia


Dendropsophus minutus is a hylid widely distributed in the Neotropics. Although this species is classified as «least concern», some populations are being affected by the advance of various anthropic activities. Despite previous studies on the biology of the species, some aspects of its trophic ecology are still unknown. We used stomach content analysis (SCA) and stable isotope analysis (SIA) to identify diet, trophic position and the primary food sources and preys that support a post-metamorphic population of D. minutus in the coastal plain of extreme southern Brazil. SCA revealed that Diptera (%PSIRI = 10.16), Hemiptera (%PSIRI = 5.83), and Araneae (%PSIRI = = 1.46) were present in the diet. The primary-producers-based mixing model indicated POM (particulate organic matter) as the main basal carbon source sustaining the analyzed specimens; whereas the prey-based mixing model showed that Hemiptera is the main resource assimilated by the species. Mean trophic position was 2.71, ranging between 2.52 and 2.91.


treefrog; Neotropical; stable isotopes

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Atkinson C. L., Golladay S. W., and Smith L. L. (2017), «Larval anuran stable isotope signatures and stoichiometry across multiple geographically isolated wetlands in the southeastern United States», Southeastern Naturalist, 16(1), 87 – 104.

Basso N. G. (1988), «Estrategias adaptativas de una comunidad subtropical de anuros (Doctoral dissertation, Universidad Nacional de La Plata)», Ser. Monogr., 1, 1 – 70.

Bastos R. F., Corrêa F., Winemiller K. O., and Garcia A. M. (2017), «Are you what you eat? Effects of trophic discrimination factors on estimates of food assimilation and trophic position with a new estimation method», Ecol. Indicators, 75, 234 – 241.

Bivand R., Rowlingson B., Diggle P., Petris G., Eglen S., and Bivand M. R. (2017), Packagesplancs’. R package version 2, (accessed on March 15, 2022).

Brown S. C., Bizzarro J. J., Cailliet G. M., and Ebert D. A. (2012), «Breaking with tradition: redefining measures for diet description with a case study of the Aleutian skate Bathyraja aleutica (Gilbert 1896)», Environm. Biol. Fishes, 95, 3 – 20.

Carreira S. and Maneyro R. (2019). Libro Rojo de los Anfibios y Reptiles del Uruguay. Biología y conservación de los Anfibios y Reptiles en peligro de extinción a nivel nacional, Dinama, Uruguay

Carvalho W. F., Franco F. C., Godoy F. R., Folador D., Avelar J. B., Nomura F., Divino da Cruz A., Sabóia-Morais S. M. T., Bastos R. P., and Silva D. D. M. (2018), «Evaluation of genotoxic and mutagenic effects of glyphosate roundup original® in Dendropsophus minutus Peters, 1872 tadpoles», S. Am. J. Herpetol., 13(3), 220 – 229.

Caut S., Angulo E., Díaz-Paniagua C., and Gomez-Mestre I. (2013), «Plastic changes in tadpole trophic ecology revealed by stable isotope analysis», Oecologia, 173, 95 – 105.

Cloyed C. S., Newsome S. D., and Eason P. K. (2015) «Trophic discrimination factors and incorporation rates of carbon- and nitrogen-stable isotopes in adult green frogs, Lithobates clamitans», Physiol. Biochem. Zool., 88(5), 576 – 585.

Davis N. D., Myers K. W., and Ishida Y. (1998), «Caloric value of high-seas salmon prey organisms and simulated salmon ocean growth and prey consumption», N. Pacific Anadromous Fish Commission Bull., 1, 146 – 162.

Fernandez C. N., Robe L. J., and Bugoni L. (2021), «Diet and trophic niche overlap among a native waterbird and two non-native herbivores in Pampas grasslands», Food Webs, 28, e00201.

Fry B. (2006), Stable Isotope Ecology, Springer, USA.

Frost D. R. (2023), Amphibian Species of the World: an Online Reference. Version 6.1, (accessed on April 01, 2023).

Peterson B. J. and Fry B. (1987), «Stable isotopes in ecosystem studies», Annu. Rev. Ecol. Syst., 18(1), 293 – 320.

Gehara M., Crawford A. J., Orrico V. G., Rodriguez A., Loetters S., Fouquet A., and Koehler J. (2014), «High levels of diversity uncovered in a widespread nominal taxon: continental phylogeography of the Neotropical tree frog Dendropsophus minutus», PloS one, 9, e103958.

Glibert P. M., Middelburg J. J., McClelland J. W., and Vander Zanden M. J. (2019), «Stable isotope tracers: Enriching our perspectives and questions on sources, fates, rates, and pathways of major elements in aquatic systems», Limnol. Oceanogr., 64(3), 950 – 981.

Gonçalves M. W., Vieira T. B., Maciel N. M., Carvalho W. F., Lima L. S. F., Gambale P. G., Cruz A. D., Nomura F., Bastos R. P., and Silva D. M. (2015), «Detecting genomic damages in the frog Dendropsophus minutus: preserved versus perturbed areas», Environm. Sci. Pollution Res., 22, 3947 – 3954.

Hammer Ø. (2023), PAST — PAleontological STatistics. Version 4.13. Reference Manual, (accessed on May 10, 2023).

Huckembeck S., Loebmann D., Albertoni E. F., Hefler S. M., Oliveira M. C., and Garcia A. M. (2014), «Feeding ecology and basal food sources that sustain the Paradoxal frog Pseudis minuta: a multiple approach combining stomach content, prey availability, and stable isotopes», Hydrobiologia, 740, 253 – 264.

Huckembeck S., Winemiller K. O., Loebmann D., and Garcia A. M. (2018), «Trophic ecology of two sympatric frogs with contrasting morphology and habitat use in a subtropical wetland», Herpetologica, 74(3), 207 – 216.

Huckembeck S., Winemiller K. O., Loebmann D., and Garcia A. M. (2020), «Trophic structure of frog assemblages in coastal habitats in southern Brazil», Austral. Ecol., 45(7), 977 – 989.

Layman C. A., Araujo M. S., Boucek R., Hammerschlag-Peyer C. M., Harrison E., Jud Z. R., Matich P., Rosenblatt A. E., Vaudo J. J., Yeager L. A., Post D. M., and Bearhop S. (2012), «Applying stable isotopes to examine food-web structure: an overview of analytical tools», Biol. Rev., 87(3), 545 – 562.

Leivas P. T., Mayer T. B., Leivas F. W., and Fávaro L. F. (2018a) «Trophic niche of Dendropsophus minutus (Anura: Hylidae) in southern Brazil», Phyllomedusa J. Herpetol., 17(2), 267 – 272.

Leivas P. T., Mayer T. B., and Fávaro L. F. (2018b), «The reproductive biology of Dendropsophus minutus (Amphibia: Anura) in South of Brazil», Herpetol. Notes, 11, 395 – 403.

Loebmann D. and Vieira J. P. (2005), «Relaçăo dos anfíbios do Parque Nacional da Lagoa do Peixe, Rio Grande do Sul, Brasil», Rev. Brasil. Zool., 22(2), 339 – 341.

Lopes A., Benvindo-Souza M., Carvalho W. F., Nunes H. F., Lima P. N., Costa M. S., Benetti E. J., Guerra V., Sabóia-Morais S. M. T., Santos C. E., Simões K., Bastos R. P., and Silva D. D. M. (2021), «Evaluation of the genotoxic, mutagenic, and histopathological hepatic effects of polyoxyethylene amine (POEA) and glyphosate on Dendropsophus minutus tadpoles», Environm. Poll., 289, 117911.

Macale D., Vignoli L., and Carpaneto G. M. (2008), «Food selection strategy during the reproductive period in three syntopic hylid species from a subtropical wetland of north-east Argentina», Herpetol. J., 18(1), 49 – 58.

Maneyro R. and Carreira S. (2012), Guía de anfíbios del Uruguay, Ediciones de la Fuga, Uruguay

Menin M., Rossa-Feres D. D. C., and Giaretta A. A. (2005), «Resource use and coexistence of two syntopic hylid frogs (Anura, Hylidae)», Rev. Brasil. Zool., 22(1), 61 – 72.

Morais A. R., Batista V. G., Gambale P. G., Signorelli L., and Bastos R. P. (2012), «Acoustic communication in a Neotropical frog (Dendropsophus minutus): vocal repertoire, variability and individual discrimination», Herpetol. J., 22(4), 249 – 257.

Oliveira M., Dalzochio M. S., and Tozetti A. M. (2019), «Prey selection by anurans in subtemperate swamps of the extreme south of Brazil», S. Am. J. Herpetol., 14(3), 204 – 212.

Orrico V. G., Grant T., Faivovich J., Rivera-Correa M., Rada M. A., Lyra M. L., and Haddad C. F. (2021), «The phylogeny of Dendropsophini (Anura: Hylidae: Hylinae)», Cladistics, 37(1), 73 – 105.

Parnell A. and Inger R. (2016), Simmr: a Stable Isotope Mixing Model. R package version 0.3, (accessed on April 01, 2022).

Parnell A. C., Inger R., Bearhop S., and Jackson A. L. (2010), «Source partitioning using stable isotopes: coping with too much variation», PloS one, 5, e9672.

Pebesma E. and Bivand R. S. (2005), «S classes and methods for spatial data: the sp package», R news, 5(2), 9 – 13.

Peterson B. J. and Fry B. (1987), «Stable isotopes in ecosystem studies», Annu. Rev. Ecol. Syst., 18(1), 293 – 320.

Phillips D. L., Inger R., Bearhop S., Jackson A. L., Moore J. W., Parnell A. C., Semmens B. X., and Ward E. J. (2014), «Best practices for use of stable isotope mixing models in food-web studies», Can. J. Zool., 92(10), 823 – 835.

Quezada-Romegialli C., Jackson A. L., Hayden B., Kahilainen K. K., Lopes C., and Harrod C. (2018), «tRophicPosition, an R package for the Bayesian estimation of trophic position from consumer stable isotope ratios», Meth. Ecol. Evol., 9(6), 1592 – 1599.

Silvano D., Azevedo-Ramos C., La Marca E., Coloma L. A., Ron S., Langone J., Baldo D., and Hardy J. (2010), Dendropsophus minutus, The IUCN Red List of Threatened Species, DOI: 10.2305/

Smith J. A., Mazumder D., Suthers I. M., and Taylor M. D. (2013), «To fit or not to fit: evaluating stable isotope mixing models using simulated mixing polygons», Meth. Ecol. Evol., 4(7), 612 – 618.

Vander-Zanden M. J. and Rasmussen J. B. (2001), «Variation in δ15N and δ13C trophic fractionation: implications for aquatic food web studies», Limnol. Oceanogr., 46(8), 2061 – 2066.

Villamarín F., Jardine T. D., Bunn S. E., Marioni B., and Magnusson W. E. (2018) «Body size is more important than diet in determining stable-isotope estimates of trophic position in crocodilians», Sci. Rep., 8(1), 1 – 11.

Whiles M. R., Gladyshev M. I., Sushchik N. N., Makhutova O. N., Kalachova G. S., Peterson S. D., and Regester K. J. (2010), «Fatty acid analyses reveal high degrees of omnivory and dietary plasticity in pond-dwelling tadpoles», Freshwater Biol., 55(7), 1533 – 1547.



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