Open Access Open Access  Restricted Access Subscription or Fee Access

Effect of Antibiotics Metranidazole, Streptomycin, and Gentamicin on the Maintenance of Sperm Motility of the European Common Frog (Rana temporaria) During Refrigerated Storage

Svetlana Anatolievna Kaurova, Victor Konstantinovich Uteshev, Natalia Vladimirovna Shishova

Abstract


The problem of asynchrony in the production of gametes in amphibians can be solved by increasing the longevity of spermatozoa during the storage of spermic urine with antibiotics. This study evaluated the effect of metranidazole and streptomycin at concentrations of 0.1, 0.2, 0.4, and 1 mg/ml on the maintenance of motility and the longevity of spermatozoa in the spermic urine of the European common frog (Rana temporaria) during refrigerated storage at 4°C. Metranidazole did not improve the lifespan of spermatozoa, and streptomycin had a positive effect at all analyzed concentrations. In addition, the streptomycin and gentamicin at the same concentrations of 0.05, 0.1, and 0.2 mg/ml were compared for their effect on maintaining sperm motility during storage. In spermic urine samples with significant bacterial contamination, no significant differences were found in the effect of streptomycin and gentamicin on sperm motility. In the absence of any visible signs of significant bacterial contamination of spermic urine samples, the motility of spermatozoa with gentamicin remained significantly longer than that with streptomycin for all analyzed concentrations. In addition, in this study, for the first time, a positive correlation was revealed between the initial concentration of spermatozoa and the longevity of spermatozoa during the refrigerated storage of amphibian spermic urine samples with antibiotics (r = 0.899, P < 0.0001). However, no relationship was found between the longevity of spermatozoa and the initial osmolality of spermic urine in the range from 62 to 92 mosmol/kg (r = 0.083, P = 0.768).

Keywords


Amphibian; spermatozoa; reproductive technologies, captive breeding, conservation biology

Full Text:

PDF

References


Alavi S. M. H. and Cosson J. (2005), «Sperm motility in fishes: I. Effects of temperature and pH», Cell Biol. Int., 29(2), 101 – 110. DOI: 10.1016/j.cellbi.2004.11.021

Alavi S. M. H. and Cosson J. (2006), «Sperm motility in fishes. (II) Effects of ions and osmolality: a review», Cell Biol. Int., 30(1), 1 – 14. DOI: 10.1016/j.cellbi.2005.06.004

Ananjeva N. B., Uteshev V. K., Orlov N. L., Ryabov S. A., Gakhova E. N., Kaurova S. A., Kramarova L. I., Shishova N. V., and Browne R. K. (2017), «Comparison of the modern reproductive technologies for amphibians and reptiles», Russ. J. Herpetol., 24(4), 275 – 290. DOI: 10.30906/1026-2296-2019-24-4-275-290

Arregui L., Kouba A. J., Germano J. M., Barrios L., Moore M., and Kouba C. K. (2022), «Fertilization potential of cold-stored Fowler’s toad (Anaxyrus fowleri) spermatozoa: temporal changes in sperm motility based on temperature and osmolality», Reprod. Fertil. Dev., 34(5), 461 – 469. DOI: 10.1071/RD21037

Bozkurt T., Türk G., and Gür S. (2007), «The Time-Dependent Motility and Longevity of Stallion Spermatozoa Diluted in Different Spermatozoal Concentrations and Extenders during Cool-Storage», Revue Méd. Vét., 158(02), 67 – 72. https://www.researchgate.net/publication/248391621.

Browne R. K., Clulow J., and Manony M. (2001), «Short-term storage of cane toad (Bufo marinus) gametes», Reproduction, 121, 167 – 173. DOI: 10.1530/rep.0.1210167.

Browne R. K., Clulow J., and Manony M. (2002), «The short-term storage and cryopreservation of spermatozoa from hylid and myobatrachid frogs», Cryo Lett., 23(2), 129 – 136. PMID: 12050781.

Browne R. K., Kaurova S. A., Uteshev V. K., Shishova N. V., McGinnity D., Figiel C. R., Mansour N., Agney D., Wu M., Gakhova E. N., Dzyuba B., and Cosson J. (2015), «Sperm motility of externally fertilizing fish and amphibians», Theriogenology, 83(1), 1 – 13. DOI: 10.1016/j.theriogenology.2014.09.018

Browne R. K., Kaurova S. A., Vasudevan K., McGinnity D., Venu G., Gonzalez M., Uteshev V. K., and Marcec-Greaves R. (2022), «Reproduction technologies for the sustainable management of Caudata (salamander) and Gymnophiona (caecilian) biodiversity», Reprod. Fertil. Dev., 34(6), 479 – 497. DOI: 10.1071/RD21356

Browne R. K., Silla A. J., Upton R., Della-Togna G., Marcec-Greaves R., Shishova N. V., Uteshev V. K., Proaño B., Pérez O. D., Mansour N., Kaurova S. A., Gakhova E. N., Cosson J., Dyzuba B., Kramarova L. I., McGinnity D., Gonzalez M., Clulow J., and Clulow S. (2019), «Sperm collection and storage for the sustainable management of amphibian biodiversity», Theriogenology, 133(8), 187 – 200. DOI: 10.1016/j.theriogenology.2019.03.035

Burakova A. V., Baitimirova E. A., Kshnyasev I. A., and Zubarev I. V. (2019), «Urinal Sperm Motility and Parasite Load in Anuran Amphibians (Anura)», Russ. J. Herpetol., 26(2), 69 – 76. DOI: 10.30906/1026-2296-2019-26-2-69-76

Byrne P. G., Dunne C., Munn A. J., and Silla A. J. (2015), «Environmental osmolality influences sperm motility activation in an anuran amphibian», J. Evol. Biol., 28(3), 521 – 534. DOI: 10.1111/jeb.12584

Calatayud N. E., Jacobs L. E., Williams C. L., Steiner C. C., and Shier D. M. (2022), «Recovering an endangered frog species through integrative reproductive technologies», Theriogenology, 191, 141 – 152. DOI: 10.1016/j.theriogenology.2022.07.003

Clulow J., Upton R., Trudeau V. L., and Clulow S. (2019) «Amphibian assisted reproductive technologies: moving from technology to application», in: P. Comizzoli, J. Brown, W. Holt (eds.), Reproductive Sciences in Animal Conservation. Advances in Experimental Medicine and Biology, Springer, Cham., 1200, pp. 413 – 463. DOI: 10.1007/978-3-030-23633-5_14

Colombo B. M., Scalvenzi T., Benlamara S., and Pollet N. (2015), «Microbiota and mucosal immunity in amphibians», Front. Immunol., 6, e111. DOI: 10.3389/fimmu.2015.00111

Dione N., Khelaifia S., Lagier J. C., and Raoult D. (2015), «The aerobic activity of metronidazole against anaerobic bacteria», Int. J. Antimicrob. Agents, 45(5), 537 – 540. DOI: 10.1016/j.ijantimicag.2014.12.032

Ďuračka M., Belić L., Tokárová K., Žiarovská J., Kačániová M., Lukáč N., and Tvrdá E. (2021), «Bacterial communities in bovine ejaculates and their impact on the semen quality», Syst. Biol. Reprod. Med., 67(6), 438 – 449. DOI: 10.1080/19396368.2021.1958028

Fraczek M., Szumala-Kakol A., Jedrzejczak P. M. D., Kamieniczna M., and Kurpisz M. M. D. (2007), «Bacteria trigger oxygen radical release and sperm lipid peroxidation in in vitro model of semen inflammation», Fertil. Steril., 88(4), 1076 – 1085. DOI: 10.1016/j.fertnstert.2006.12.025

Germano J. M., Arregui L., and Kouba A. J. (2013), «Effects of aeration and antibiotics on short-term storage of Fowler’s toad (Bufo fowleri) sperm», Aquaculture, 396 – 399, 20 – 24. DOI: 10.1016/j.aquaculture.2013.02.018

Gundogan M., Yeni D., Avdatek F., and Fidan A. F. (2010), «Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage», Anim. Reprod. Sci., 122, 200 – 207. DOI: 10.1016/j.anireprosci.2010.08.012

IUCN (2022), The IUCN Red List of Threatened Species. Version 2022-2; https://www.iucnredlist.org (accessed February 8, 2022).

Kaurova S. A., Browne R. K., and Uteshev V. K. (2022), «Antibiotics for the refrigerated storage at 4°C of hormonally induced European common frog (Rana temporaria) spermatozoa», Theriogenol. Wild, 1, e100009. DOI: 10.1016/j.therwi.2022.100009

Keogh L. M., Byrne P. G., and Silla A. J. (2017), «The effect of gentamicin on sperm motility and bacterial abundance during chilled sperm storage in the Booroolong frog», Gen. Comp. Endocrinol., 243, 51 – 59. DOI: 10.1016/j.ygcen.2016.11.005

Kidov A. A., Matushkina K. A., Uteshev V. K., Timoshina A. L., and Kovrina E. G. (2014), «The first captive breeding of the Eichwald’s toad (Bufo eichwaldi)», Russ. J. Herpetol., 21(1), 40 – 46. DOI: 10.30906/1026-2296-2014-21-1-40-46

Kocabaş M., Kutluyer F., Ertekin Ö., Aksu Ö., and Başçınar N. (2019), «Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan», Syst. Biol. Reprod. Med., 65(3), 187 – 193. DOI: 10.1080/19396368.2019.1566414

Langhorne C. J., Calatayud N. E., Kouba C. K., Willard S. T., Smith T., Ryan P. L., and Kouba A. J. (2021), «Efficacy of hormone stimulation on sperm production in an alpine amphibian (Anaxyrus boreas boreas) and the impact of short-term storage on sperm quality», Zoology, 146, e125912. DOI: 10.1016/j.zool.2021.125912

Matushkina K. A., Kidov A. A., and Litvinchuk S. N. (2020), «Keeping, breeding and maintenance of zooculture of the Ladakh toad, Bufotes latastii (Boulenger, 1882)», Russ. J. Herpetol., 27(5), 284 – 290. DOI: 10.30906/1026-2296-2020-27-5-284-290

McGinnity D., Reinsch S. D., Schwartz H., Trudeau V., and Browne R. K. (2022), «Semen and oocyte collection, sperm cryopreservation and IVF with the threatened North American giant salamander Cryptobranchus alleganiensis», Reprod. Fertil. Dev., 34(5), 470 – 477. DOI: 10.1071/RD21035

Moretti E., Capitani S., Figura N., Pammolli A., Federico M. G., Giannerini V., and Collodel G. (2009), «The presence of bacteria species in semen and sperm quality», Assist. Reprod. Gen., 26(1), 47 – 56. DOI: 10.1007/s10815-008-9283-5

Rocha-Garduño G., Hernández-Martínez N. A., Colín-Lozano B., Estrada-Soto S., Hernández-Núñez E., Prieto-Martínez F. D., Medina-Franco J. L., Chale-Dzul J. B., Moo-Puc R., and Navarrete-Vázquez G. (2020), «Metronidazole and Secnidazole Carbamates: Synthesis, Antiprotozoal Activity, and Molecular Dynamics Studies», Molecules, 25(4), e793. DOI: 10.3390/molecules25040793

Rudin-Bitterli T. S., Mitchell N. J., and Evans J. P. (2020), «Extensive geographical variation in testes size and ejaculate traits in a terrestrialbreeding frog», Biol. Lett., 16, e20200411. DOI: 10.1098/rsbl.2020.0411

Silla A. J. (2013), «Artificial fertilisation in a terrestrial toadlet (Pseudophryne guentheri): effect of medium osmolality, sperm concentration and gamete storage», Reprod. Fertil. Dev., 25(8), 1134 – 1141. DOI: 10.1071/RD12223

Silla A. J., Keogh L. M., and Byrne P. G. (2015). «Antibiotics and oxygen availability affect the short-term storage of spermatozoa from the critically endangered booroolong frog, Litoria booroolongensis», Reprod. Fertil. Dev., 27(8), 1147 – 1153. DOI: 10.1071/RD14062

Silla A. J., Keogh L. M., and Byrne P. G. (2017), «Sperm motility activation in the critically endangered booroolong frog: the effect of medium osmolality and phosphodiesterase inhibitors», Reprod. Fertil. Dev., 29(11), 2277 – 2283. DOI: 10.1071/RD17012

Togna G. D., Howell L. G., Clulow J., Langhorne C. J., Marcec-Greaves R., and Calatayud N. E. (2020), «Evaluating amphibian biobanking and reproduction for captive breeding programs according to the Amphibian Conservation Action Plan objectives», Theriogenology, 150, 412 – 431. DOI: 10.1016/j.theriogenology.2020.02.024

Tuset V. M., Dietrich G. J., Wojtczak M, Słowińska M., De Monserrat J., and Ciereszko A. (2008), «Relationships between morphology, motility and fertilization capacity in rainbow trout (Oncorhynchus mykiss) spermatozoa», J. Appl. Ichthyol., 24(4), 393 – 397. DOI: 10.1111/j.1439-0426.2008.01145.x

Uteshev V. K., Kaurova S. A., Shishova N. V., Stolyarov S. D., Browne R. K., and Gakhova E. N. (2015), «In vitro fertilisation with hormonally induced sperm and eggs from sharp-ribbed newts Pleurodeles waltl», Russ. J. Herpetol., 22(1), 35 – 40. DOI: 10.30906/1026-2296-2015-22-1-35-40

Uteshev V. K, Gakhova E. N, Kramarova L. I., Shishova N. V., Kaurova S. A., Kidova E. A., Kidov A. A., and Browne R. K. (2023), «Russian Collaborative Development of Reproduction Technologies for the Sustainable Management of Amphibian Biodiversity», Asian Herpetol. Res., 14(1), 103 – 115. DOI: 10.16373/j.cnki.ahr.220043

Waters M. and Tadi P. (2022), Streptomycin, StatPearls Publishing, Treasure Island (FL), https://www.ncbi.nlm.nih. gov/books/NBK555886 (accessed July 11, 2022).

Weir C. B. and Le J. K. (2020), Metronidazole, StatPearls Publishing LLC, https://www.ncbi.nlm.nih.gov/books/ NBK539728 (accessed February 12, 2023).

Yaniz J. L., Marco-Aguado M. A., Mateos J. A., and Santolaria P. (2010), «Bacterial contamination of ram semen, antibiotic sensitivities, and effects on sperm quality during storage at 15 C», Anim. Rep. Sci., 122, 142 – 149. DOI: 10.1016/j.anireprosci.2010.08.006




DOI: https://doi.org/10.30906/1026-2296-2024-31-1-47-55

Refbacks

  • There are currently no refbacks.


You can subscribe to the print or electronic version of the journal on the site of EastView Company. If you have any questions, please write to the email sales@ivis.ru