On the Hybridogenous Origin of the Parthenogenetic Species Lacerta dahli and Lacerta rostombekovi Revealed by RAPD Technique

N. L. Ryabinina, V. V. Grechko, S. K. Semenova, Ilya S. Darevsky

Abstract


The hypothesis of hybridogenous origin of lacertid parthenogenetic species was verified by RAPD method with several arbitrary primers. The hypothesis appeared to be correct as both parthenogenetic lizard DNAs contain only the RAPD loci of putative parental bisexual species and practically no ones specific for parthenospecies. The most probable parentals are L. mixta and L. portschinskii for L. dahli, and some of the populations of L. raddei/nairensis complex and L. portschinskii, too, for L. rostombekovi.

Keywords


parthenogenesis; hybridogenesis; lizards; RAPD; DNA

Full Text:

PDF

References


Blin N. and Stafford D. W. (1976), «A general method for isolation of high molecular weight DNA from eucaryotes», Nucleic Acids Res., 3(9), 2303–2308.

Bobin M. L., Darevsky I. S., Kupriyanova L. A., MacCulloch R. D., Upton D. E., Danielyan F. D., and Murphy R. W. (1996), «Allozyme variation in populations of Lacerta raddei and L. nairensis (Sauria: Lacertidae) from Armenia», Amphibia–Reptilia, 17, 233–246.

Bolditch B. M., Albright D. G., Williams J. G. K., and Braun M. J. (1993), «Randomly amplified polymorphic DNA markers in comparative genome studies», in: Methods in Enzymology, 224, 294–309.

Bullini L. (1994), «Origin and evolution of animal hybrid species», Trends Ecol. Evol., 9, 422–426.

Darevsky I. S. (1993), «Evolution and ecology of parthenogenesis in reptiles», in: Current Research in Biology of Amphibians and Reptiles. Proc. 1st Congr. Herpetol., Soc. for Study of Amphibians and Reptiles, K. Adler (ed.), Oxford (Ohio, USA), pp. 21–39.

Darevsky I. S., Kan N. G., Ryabinina N. L., et al. (1998), «Biological and molecular genetic characteristics of the parthenogenetic lizard Lacerta armeniaca (Mehely), introduced from Armenia to Ukraine»., Dokl. Ross. Akad. Nauk, 363(6), 846–848 [in Russian].

Grechko V. V., Kataev M. V., Melnikova M. N., and Darevsky I. S. (1993), «Relations of parthenogenetic Lacerta lizards and their presumable parental bisexual species deduced from comparative data on amplification products obtained by single-primer PCR», Mol. Biol., 27, 891–897 [in Russian].

Grechko V. V., Ryabinin D. M., Fedorova L. V., et al. (1998), «DNA taxoprints of some Lacertidae lizards: taxonomic and phylogenetic implications», Mol. Biol., 32, 151–160 [in Russian].

Moritz C., Uzzell T., Spolsky H., Hotz H., Darevsky I. S., Kupriyanova L. A., and Daniyelyan F. (1992), «The maternal ancestry and approximate age of parthenogenetic species of Caucasian rock lizards (Lacertidae: Lacerta)», Genetica, 87, 53–62.

Murphy R. W., Darevsky I. S., Kupriyanova L. A., MacCulloch R. D., and Fu J. (1999), «A fine line between sex and unisexuality: the phylogenetic constraints on lacertid lizards», Evolution, in press.

Ryabinin D. M., Grechko V. V., Darevsky I. S., Ryskov A. P., and Semenova S. K. (1996), «Comparative study of DNA repetition sequences by means of restriction endonucleases among populations and subspecies of some lacertid lizard species», Rus. J. Herpetol., 3(2), 178–185.

Ryabinina N. L., Grechko V. V., and Darevsky I. S. (1998), «DNA polymorphisms of some lizards (fam. Lacertidae) as determined by RAPD technique», Genetika, 34(12), 1661–1667 [in Russian].

Uzzell T. and Darevsky I. S. (1975), «Biochemical evidence for the hybrid origin of the parthenogenetic species of the Lacerta saxicola complex (Sauria: Lacertidae) with a discussion of some ecological and evolutionary implications», Copeia, 1975, 204–222.

Vrijenhoek R. C., Dawley R. M., Cole J., and Bogart J. P. (1989), «A list of the known unisexual vertebrates», in: Evolution and Ecology of Unisexual Vertebrates, R. M. Dowley and J. P. Bogart (eds.), Bull. 466, New York State Museum, Albany (New York, USA), pp. 19–23.




DOI: https://doi.org/10.30906/1026-2296-1999-6-1-55-60

Refbacks

  • There are currently no refbacks.


You can subscribe to the print or electronic version of the journal on the site of EastView Company. If you have any questions, please write to the email sales@ivis.ru