Estimation of the Subspecific Level of Differentiation in Caucasian Lizards of the Genus Darevskia (Syn. «Lacerta saxicola complex», Lacertidae, Sauria) Using Genome DNA Markers

N. L. Ryabinina, A. A. Bannikova, S. A. Kosushkin, D. G. Ciobanu, K. D. Milto, B. S. Tuniyev, V. F. Orlova, V. V. Grechko, I. S. Darevsky


The taxonomic categories such as population and subspecies were studied on the example of three Caucasian lizard species of genus DarevskiaD. praticola, D. derjugini, and D. rudis by comparing the morphological data and results inferred from nuclear DNA markers. RAPD and new inter-MIR-PCR (IM-PCR) methods were used. The IM-PCR was used to characterize the lacertid DNA fragments located between dispersed SINE type repeats which occurred to be ortologous to mammalian repeats of the same type. It was shown that separation of the Northern population of D. derjugini (subspecies silvatica) is supported by the comparison with two Southern populations (derjugini and barani). The latter ones, in their turn, are very similar and hardly can be considered as good subspecies by the genetic distance. The subspecific division of D. praticola (praticola and pontica) also requires more specification. For example, several populations from North Caucasus (ssp. praticola) occurred to be heterogeneous. The level of differences between ssp. praticola and pontica is of the same order as in some of the praticola populations. Low level of molecular differences between two subspecies of D. rudis (obscura and bischoffi) does not confirm their validity as a full subspecies.


lizard; Lacertidae; Darevskia; systematics; RAPD; inter-MIR-PCR method; population; subspecies; species

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Bannikova A. A., Matveev V. A., and Kramerov D. A. (2002), «The experience of Inter-MIR PCR usage to study the phylogeny of mammals», Genetika [in Russian], 38(6), 1 – 12.

Bischoff W. (1982), «Zur Kenntnis der innerartlichen Gliederung der Arwin Eidechse, Lacerta derjugini Nikolskij, 1898», Zool. Abh., 38(1), 1 – 52.

Bischoff W. (1984) «Bemerkungen zur innerartlichen Gliederung und zur Verbreitung der Artwiner Eidechse (Lacerta derjugini Nikolskij, 1898) an den Sudhangen des Großen Kaukasus (Sauria: Lacertidae)», Salamandra, 20(2/3), 101 – 111.

Buntjer J. B. (1997), «DNA repeats in the vertebrate genome as probes in phylogeny and species identification», in: DNA Repeats in Vertebrate Genome as Probes in Phylogeny and Species Identification, Utrecht. Univ. Siteit. Utrecht. Mederlands, pp. 1 – 130.

Ciobanu D. G., Rudykh I. A., Ryabinina N. L., Grechko V. V., Kramerov D. A., and Darevsky I. S. (2002), «Reticular evolution of parthenogenetic species of Lacertidae rock lizards: inheritance of CLsat tandem repeats and anonymous RAPD markers», Mol. Biol. [in Russian], 36(2), 296 – 306.

Fedorov A., Fedorova L., Grechko V. V., Ryabinin D. M., Sheremet’eva V. A., Bannikova A. A., Lomov A. A., Ryskov A. P., and Darevsky I. S. (1999), «Variable and invariable DNA repeats characters revealed by taxoprint approach are useful for molecular systematics», J. Mol. Evol., 48, 69 – 76.

Gilbert W. and Labuda D. (2000), «Evolutionary inventions and continuity of CORE-SINE in mammals», J. Mol. Biol., 298, 365 – 377.

Grechko V. V. (2002), «Molecular markers of DNA to study phylogeny and systematics», Genetika [in Russian], 38(8), 1013 – 1033.

Grechko V. V., Ryabinin D. M., Fedorova L. V., Rudykh I. A., Fedorov A. N., Ryskov A. P., Semenova S. K., and Darevsky I. S. (1998) «Molecular-genetic classification and phylogenetic relationships of some lizard species (fam. Lacertidae) based on restriction sites distribution in repetitive DNA fraction (taxonoprint method)», Mol. Biol. [in Russian], 32(1), 172 – 183.

Hadrys H., Balick M., and Schierwater B. (1992), «Application of randomly amplified polymorphic DNA (RAPD) in molecular ecology». Mol. Ecol., 1, 55 – 63.

Hillis D. M. (1987), «Molecular vs. morphological approach to systematics», Annu. Rev. Ecol. Syst., 18, 23 – 42.

Orlova V. F. (1978a), «Geographical variability and description of lectotype of Artwin lizard Lacerta derjugini Nik», in: Proc. Zool. Museum of Lomonosov Moscow State University. Studies of USSR fauna. Birds and Reptiles. Vol. 17 [in Russian], pp. 188 – 203.

Orlova V. F. (1978b), «Geographical distribution and intraspecies polymorphism of meadows lizard in Caucasus», in: Proc. Zool. Museum of Lomonosov Moscow State University. Studies of USSR fauna. Birds and Reptiles. Vol. 17 [in Russian], pp. 204 – 215.

Ryabinina N. L., Grechko V. V., and Darevsky I. S. (1998), «DNA polymorphisms of some lizards (fam. Lacertidae) as determined by RAPD technique», Genetika [in Russian], 34, 1661 – 1667.

Sambrook I., Fritsch E. F., and Maniatis T. (1989), Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Lab, Cold Spring Harbor Press, New York.

Yurka J., Zietkiewicz E., and Labuda D. (1995), «Ubiquitous mammalian-wide interspersed repeats (MIRs) are molecular fossils from mesozoic era» Nucl. Acids Res., 23, 170 – 175.



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