The Histological Investigation of Liver Tissues in Triturus karelinii and Triturus vulgaris (Salamandridae, Urodela)

Yücel Başimoğlu Koca, Beyhan Gürcü, Erdal Balcan


The microscopic structures of the liver tissues from two amphibian species, T. vulgaris and T. karelinii, were studied by different histological staining techniques. The polyhedral shaped parenchymal cells of T. karelinii contain euchromatic mononuclei and dense cytoplasmic glycogen. These cells arranged as systematical cell cords in parenchyma. Pigment granules containing Kupffer cells (melanomacrophages) were found in the sinusoid wall. T. karelinii liver parenchyma have shown no clear lobulation and no concentrated reticular fiber network around the hepatocytes and vessels. The predominant glycosaminoglycan (GAG) type of T. karelinii was hyaluronic acid (HA), however, sulphated GAGs were almost absent.

Hepatocytes from T. vulgaris were not polyhedral shaped, and contain euchromatic mononucleus. The glycogen content was not significant quantities in hepatocyte cytoplasm of T. vulgaris. These cells form radial arrangenment close to large vessels, however they found as cell clusters in bottom of parenchyma. Kupffer cells were found between the parenchymal cell groups and at the sinusoidal walls. These cells contain many pigment ganules in their cytoplasms. Reticular fibre networks were found between hepatocytes and vessels in lobulation lacking parenchyma. Around the hepatocytes and vessels, hyaluronic acid was the predominant GAG type.


Triturus karelinii; Triturus vulgaris; liver histology

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Agius C. (1980), «Phylogenetic development of melanomacrophage centers in fish», J. Zool. (Lond.), 191, 11 – 31.

Baker J. M. R. and Halliday T. R. (2000), «Variation in dorsal crest morphology and tail height with age in great crested newts (Triturus cristatus)», Herpetol. J., 10, 173 – 176.

Bancroft J. D., Cook H. C., Stirling R. W., and Turner D. R. (1995), Manual of Histological Technique and Their Diagnostic Application, Churchill, Livingstone, USA.

Barni S., Vaccarone R., Bertone V., Fraschini A., Bernini F., Breckenridge W. R., Nathanaels, and Pereira L. (1987), «Some aspect of the biology and development of Ichtyophis glutinosus (Amphibia: Gymnopiana)», J. Zool. (Lond.), 211, 437 – 449.

Barni S. and Bernocchi G. (1991), «Internalization of erythrocytes into liver paranchimal cells in naturally hibernating frog (Rana esculenta L.)», J. Exp. Zool., 258, 143 – 150.

Barni S., Bernini F., Bertone V., and Barbieri F. (1994), «Modificazioni morfocitochimiche della cellula epatica di Triturus alpestris apuanus Triturus carnifex durante in periodo invernale», Studi Trendini Sci. Nat. Acta Biol., 71, 119 – 126.

Barni S., Bertone V., Croce A. C., Bottiroli G., Bernini F., and Gerzeli G. (1999), «Increase in liver pigmentation during natural hibernation in some amphibians», J. Anat., 195, 19 – 25.

Barni S., Vaccarone R., Bertone V., Fraschini A., Bernini F., and Fenoglio C. (2002), «Mechanism of changes to the liver pigmentary component during the annual cycle (activity and hibernarion) of Rana esculenta L»., J. Anat., 200, 185 – 194.

Bloom W. and Fawcett D. W. (1986), A Textbook of Histology. 11th Ed., W. B. Saunders Company, Philadelphia, pp. 679 – 708.

Boulhic M. and Gabaudan J. (1992), «Histological study of the organogenesis of the digestive system and swim bladder of the dover sole, Solea solea (Linnaeus, 1758)», Aquaculture, 102, 373–396.

Chamlian A., Benkel L., Minko D., Njee T., and Gulian J.-M. (1989), «Ultrastructural heterogenety of glycogen in human liver», Liver, 9, 346 – 350.

Cicero R., Mallardi A., Maida I., Gallone A., and Pintucci G. (1989), «Melanogenesis in the pigment cells of Rana esculenta L.: evidence for tyrosinase-like activity in the melanosome protein fraction», Pigment Cell Res., 2, 100 – 108.

Corsaro C., Scalia M., Sinatra M., and Sichel G. (1990), «Circannual rhythm of the melanin content in frog liver (Rana esculenta L.)», Pigment Cell Res., 3, 120 – 122.

Scalia C., Scalia M., Leotta N., Mondio F., and Sichel G. (2000), «Characterisation of Kupffer cells in some Amphibia», J. Anat., 196, 249 – 261.

Davies J. A., Fisher C. E., and Barnett M. W. (2001), «Glycosaminoglycans in the study of mammalian organ development», Biochem. Soc. Trans., VOL...(Pt. 2), 166 – 71.

Epperlein H. H. and Lofberg J. (1990), «The development of the larval pigment pattren in Triturus alpestris and ambystoma», Adv. Anat. Embriyol. Cell Biol., 118, 1 – 89.

Fausto N. (1990), «Hepatocyte differatiation and liver progenitor cells», Curr. Opin. Cell Biol., 2, 1036 – 1042.

Fenoglio C., Bernocchi G., and Barni S. (1992), «Frog hepatocyte modification induced by seasonal variotions: a morphological and cytochemical study», Tissue Cell, 24, 17 – 29.

Fenoglio C. (1992), Mechanism of changes to the liver pigmentary component during the annual cycle (activity and hibernation) of Rana esculenta L»., J. Anat., 200, 185 – 195.

Guida G., Maida I., Gallone A., Boffoli D., and Cicero R. (1998), «Ultrastructural and functional study of the liver pigment cells from Rana esculanta L»., In vitro Cell. Devel. Biol. Animal, 34, 339 – 400.

Iancu T. C., Lichterman L., and Neustein H. B. (1978), «Hepatic sinusoidal cells in iron overload. Ultrastructural observations», Israel J. Med. Sci., 14, 1191 – 1201.

Jared C., Navas C. A., and Toledo R. C. (1999), «An appreciation of the phisiology and morphology of the Caecilians (Amphibia: Gymnopiana)», Comp. Biochem. Physiol. A. Mol. Integr. Physiol., 123, 313 – 328.

Junqueria C. L. and Corneiro J. (1989), Basic Histology. 6th Ed., pp. 341 – 354.

Kupfer A. and Kneitz S. (2000), «Population ecology of the great crested newt (Triturus cristatus) in an agricultural landscape. Dynamics, pond fidelity and dispersal», Herpetol. J., 10, 165 – 171.

Laurent T. C. and Fraser J. R. (1986), «The properties and turnover of hyaluronan», Ciba Found Symp., 124, 9 – 29.

Laurent T. C. and Fraser J. R. (1992), «Hyaluronan», FASEB J., 6(7), 2397 – 404.

Laurent T. C., Laurent U. B., and Fraser J. R. (1996), «The structure and function of hyaluronan: An overview», Immunol. Cell Biol., 74(2), 1 – 7.

McCourt P. A., Smedrod B. H., Melkko J., and Johansson S. (1999), «Characterization of a hyaluronan receptor on rat sinusoidal liver endothelial cells and its function relationship to scavenger receptors», Hepatology, 30(5), 1276 – 86.

McCuscey R. and McCuscey P. A. (1990), «Fine structure and function of Kupffer cells», J. Electron Microsc. Techn., 14, 237 – 246.

Motta P. (1975), «A scannig electron microscopy study of rat liver sinusoid. Endothelial and Kupffer cells», Cell Tissue Res., 164, 371 – 385.

Naito M., Hasegawa G., and Takahashi K. (1997), «Development, differentiation, and maturation of Kupffer cells», Microsc. Res. Techn., 39, 350 – 364.

Richter G. W. (1978), «The iron-loaded cell. The cytopathology of iron storage», Am. J. Pathol., 91, 363 – 405.

Richter G. W. (1984), «Studies of iron overload. Rat liver siderosome ferritin», Lab. Invest., 50, 26 – 35.

Scalia M., Geremia E., Scalia C., Santro C., Sciuto S., and Sichel G. (1988), «The extracutaneous pigmentary system: Evidence for the melanosynthesis in amphibia and Reptilia liver», Comp. Biochem. Physiol. B, 89, 715 – 717.

Scalia M., Geremia E., Scalia C., Santro C., Baratta D., and Sichel G. (1988), «Lipid peroxidation in pigmented and unpigmented liver tissue: protective role of melanin», Pigment Cell Res., 3, 115 – 119.

Schmucker D. L. (1990), «Hepatocyte fine structure during maturation and senescence», J. Electron Microsc. Techn., 14, 106 – 125.

Shylaja R. and Alexander K. M. (1974), «Studies on the physiolgy of excretion the fresh water prosobransh, pila virens: part I Pattren of excretion in the normal and post aestivating. P. Virens», Indian J. Exp. Biol., 13, 363 – 365.

Sichel G., Brai M., Palminteri M. C., and Sciuto S. (1981), Seasonal depence of ESR features of frog melanins», Comp. Biochem. Physiol. B, 70, 611 – 613.

Sichel G. (1981), «Biosynthesis and function of melanins in hepatic pigmentary system», Pigment Cell Res., 1, 250 – 258.

Sichel G., Scalia C., Cassini E., Magnani C., and Bolognani L. (1994), «ATPase activity of melanosomes in liver pigment cells of Rana esculenta L»., Comp. Biochem. Physiol., 108, 521 – 528.

Sichel G., Scalia M., Mondio F., and Scalia C. (1997), «The amphibian Kupffer cells build and demolish melanosomes: an ultrastructural point of view», Pigment Cell Res., 10, 271 – 287.

Tohoku J. (2001), «Changes in glycosaminoglycan, galactosyltransferase-I, and sialyltransferase during rat liver regeneration», Exp. Med., 193(3), 197 – 95.

Toledo R. C. and Jared C. (1995), «Cutaneous granular glands and amphibian cenoms», Comp. Biochem. Physiol., 11, 1 – 29.

Wake M. H. and Hanken J. (1982), «Development of the skull of Dermophis mexianus (Amphibia: Gymnophiona), with comments on skull and amphibian relationships», J. Morphol., 73, 203 – 222.

Windmann J. J., Cotran R. S., and Fahimi H. D. (1972), «Mononuclear phagocytes (Kupffer cells) and endothelial cells», J. Cell Biol., 52, 159 – 170.

Windmann J. J. and Fahimi H. D. (1975), «Proliferation of mononuclear phagocytes (Kupffer cells) endothelial cells», Am. J. Pathol., 80, 349 – 366.

Wisse E. (1977), «Ultrastructure and function of Kupffer cells and other sinusoidal cells in the liver», in: E. Wisse and D. L. Knook (eds.), Kupffer Cells and Other Liver Sinusoidal Cells, Elsevier – North-Holland Biomed. Press, Amsterdam, pp. 33 – 60.

Wisse E. (1980), «On the fine structure and function of rat liver Kupffer cells», in: Carr I. and Deams W. T. (eds.), The Reticuloendothelial System: A Comprehensive Treatise. Vol. 1, Plenum Press, New York, pp. 361 – 379.

Zuasti A., Ferrer C., Aroca P., and Solono F. (1990), «Distribution of extracutaneous melanin pigment in Sparus auratus, Mugil cephalus, and Dicentrarchus labrax (Pices, Teleostei)», Pigment Cell Res., 3, 126 – 131.

Zapata A., Gamariz R. P., Garrido E., and Cooper E. L. (1982), Lymphoid organs and blood cells of the Caecilian Ichthyophis kohtaonensis», Acta Zool., 63, 11 – 16.



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