Hematological and Immunological Mechanisms of Adaptation to Hibernation in Common Indian Toad Duttaphrynus melanostictus

Suman Pratihar, Jayanta Kumar Kundu

Abstract


Some hematological parameters of the hibernating and non hibernating toads Duttaphrynus melanostictus have been investigated. The immunoglobulin IgM amount, total red blood cell and neutrophil counts were significantly less (p < 0.05) in the hibernating toads as compared to the non hibernating ones. On the contrary, total white blood cell, eosinophil and lymphocyte counts were significantly more (p < 0.05) in hibernating individuals. No significant difference in the monocyte amount was observed. Hematological and immunological mechanisms of adaptation to hibernation in amphibians are discussed.


Keywords


Duttaphrynus melanostictus; hibernation; blood cells; IgM; SDS-PAGE

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References


Churchill T. A. and Storey K. B. (1993), «Dehydration tolerance in wood frogs: a new perspective on development of amphibian freeze tolerance», Am. J. Physiol., 265, 1324 – 1332.

Dupasquier L., Courtet M., and Mussmann R. (2000), «B-cell development in amphibian Xenopus», Immunol. Rev., 175, 201 – 213.

Edwards J. R., Jenkins J. L., and Swanson D. L. (2004), «Seasonal effects of dehydradation on glucose mobilization in freeze tolerant chorus frogs (Pseudacris triseriata)», J. Exp. Zool., 301A, 521 – 531.

Forbes M. R., Mcrver D. L., and Shutler D. (2006), «White blood cell profile of breeding American toads (Bufo americanus) relative to sex and body size», J. Comp. Clin. Pathol., 15, 155 – 159.

Holzapfel R. A. (1998), «The cyclic character of hibernation in frogs», Quart. Rev. Biol., 12(1), 65 – 84.

Janeway C. A., Travers P., Walport M., and Schlomchik M. J. (2001), Immunobiology: The Immune System in Health and Disease, 5th Edition, Garland Publishing, New York.

Laemlii U. K. (1970), «Cleavage of structural proteins during the assembly of the head of Bacteriophage T4», Nature, 227, 680 – 685.

Lowry O. H., Brough N. J. R., Farr A. L., and Randall R. J. (1951), «Protein measurement with the Folin phenol reagent», J. Biol. Chem., 193, 265 – 275.

Raffel T. R., Rohr J. R., Kiesecker J. M., and Hudson P. J. (2006), «Negative effects of changing temperature on amphibian immunity under field conditions», Func. Ecol., 20, 819 – 828.

Storey K. B. (2000), «Turning down the fire of life: Metabolic regulation of hibernation and estivation», in: BIOS Scientific Publication, Oxford, pp. 1 – 2.

Wojtaszek J. and Adamowicz A. (2003), «Hematology of fire-bellied toad, Bombina bombina», J. Comp. Clin. Pathol., 12, 129 – 134.




DOI: https://doi.org/10.30906/1026-2296-2010-17-2-97-100

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